牛樟芝對骨組織生成的影響

牛樟芝對骨組織生成的影響

 林群智1 劉威忠2 郭倩妤2 黃雅令1 郭心語2 耿念慈2*

1南華大學自然醫學所

2慈濟技術學院放射系、通識教育中心

摘要

牛樟芝（Antrodia cinnamomea）為生長在台灣特有樹種-牛樟上之蕈類，目前常被用於治療肝病、腹瀉、腹痛、高血壓以及腫瘤，具有抗氧化、抗發炎及抗癌之功效。然而，牛樟芝對骨組織生成的影響甚少被探討，本實驗主要目的在探討牛樟芝的萃取液對骨組織細胞分化作用的影響與其可能的機轉。

       本研究以牛樟芝萃取液（1 μg/mＬ）培養小鼠造骨先驅細胞，3天後，抽取細胞核醣核酸，進行核結合蛋白因子（Cbfa）與破骨細胞分化因子（ODF）的基因表現分析。細胞培養7天後，收集培養液培養分離自人類週邊血液之幹細胞，觀察對破骨細胞分化的影響，另於第10天統計造骨細胞數目。

       結果顯示，萃取物可增加造骨細胞分化、抑制破骨細胞的數目並可增加Cbfa及抑制ODF的基因表現。由以上結果推論，牛樟芝萃取物可促進骨組織生成，其機轉可能透過增加Cbfa基因表現量及降低ODF基因表現量來達成。

 

關鍵字：牛樟芝，幹細胞，造骨細胞，破骨細胞

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Effects of antrodia cinnamomea on bone tissue formation

 C.C. Lin1  W.C. Liu2  Q.Y. Kuok 2  Y.L. Huang1  H.Y. Kuo2  N.T. Keng1*

 1 Institute of Natural Healing Science, Nanhua University

 2 Department of Radiological Technology/General Education Center,

 Tzu-Chi College of Technology

 Abstract

 Niuchangchih is a fungus only growing on an endemic species, Cinnamomum kanehirae Hayata, of . Niuchangchih has the efficacies of anti-oxidation, anti-inflammation and anti-cancer, which is presently applied to treat liver diseases, diarrhea, abdominal pain, hypertension and tumorigenic diseases. However, the effect of niuchangchih on bone growth is seldom probed. This study aimed to investigate the effect of niuchangchih extract on cell differentiation of bone tissues as well as the possible mechanisms.

 In this research, niuchangchih extract（1 μg/mL）was used to culture osteoblast progenitor cells of mice. After 3 days, the RNA of cultured cells was sampled for gene expression analysis of core binding factors α（Cbfa）and osteoclast differentiation factor（ODF）. The cultured medium was collected after 7 days to culture the stem cells separated from human peripheral blood and to investigate its effect on the differentiation of osteoclasts. The number of osteoblasts was also counted on the tenth day.

 The results indicated that the niuchangchih extract was capable of increasing osteoblast differentiation, inhibiting the number of osteoclasts, as well as stimulating gene expression of Cbfa and suppressing that of ODF. Accordingly, the niuchangchih extract was suggested able to enhance the formation of bone tissues via the mechanisms of increasing gene expression of Cbfa and suppressing that of ODF.

 Keywords：niuchangchih, stem cell, osteoclast, osteoblast

 

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